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ASRGL1 Monoclonal Antibody

  • 中文名稱：
    ASRGL1 Monoclonal Antibody
  • 貨號：
    CSB-MA786347
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of ASRGL1 expression in HeLa cells using ASRGL1 antibody. Green, isotype control; red, ASRGL1.
    • Validation of ASRGL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ASRGL1 antibody and analyzed using BD flow cytometer.
    • Western blotting analysis using ASRGL1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ASRGL1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
    • Western blotting analysis using ASRGL1 antibody. ASRGL1 expression in wild-type (WT) and ASRGL1 shRNA knockdown (KD) HeLa cells with 20μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ASRGL1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • 其他：

產品詳情

  • 產品名稱：
    Mouse anti-Homo sapiens (Human) ASRGL1 Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    ASRGL1; Asparaginase And Isoaspartyl Peptidase 1; ALP; ALP1; Isoaspartyl Peptidase/L-Asparaginase; Asparaginase-Like 1 Protein; L-Asparagine Amidohydrolase; Asparaginase-Like Protein 1; Beta-Aspartyl-Peptidase; Isoaspartyl Dipeptidase; Asparaginase Like 1; EC 3.5.1.1; FLJ22316; CRASH; Testis Secretory Sperm-Binding Protein Li 242mP; L-Asparaginase; EC 3.4.19.5
  • 宿主：
    Mouse
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human ASRGL1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG2a
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    9C12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:400-1:2000
    FC 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.
  • 基因功能參考文獻：
    1. ASRGL1 was closely associated with growth and apoptosis in cervical cancer. Therefore, ASRGL1 may be a novel, potentially effective anticervical cancer therapy. PMID: 29767260
    2. A panel including p53 and ASRGL1 immunohistochemistry was identified as the most accurate predictor of relapse-free and disease-specific survival. Within this panel, patients were allocated into high- (5.9%), intermediate- (29.5%) and low- (64.6%) risk groups where high-risk patients had a 30-fold risk (P<0.001) of dying of EEC compared to the low-risk group. PMID: 29486992
    3. In a prospective setting ASRGL1 validates as a strong prognostic biomarker in endometrial carcinoma. Loss of ASRGL1 is associated with aggressive disease and poor survival. PMID: 29096882
    4. Our studies suggest that the p.G178R mutation in ASRGL1 leads to photoreceptor degeneration resulting in progressive vision loss. PMID: 27106100
    5. Multiple negatively charged small molecules interact within the active site of ASRGL1 to act as a base in promoting cleavage. PMID: 26780688
    6. Reduced expression of ASRGL1, defined as <75% positively stained tumor cells, was significantly associated with poor prognosis and reduced disease-specific survival in endometrioid endometrial adenocarcinoma (EEA). PMID: 25858696
    7. Determined is the structure of the first mammalian plant-type asparaginase in both a precursor and fully activated form. PMID: 22891768
    8. Human ALP cDNA was subsequently cloned. It showed 77% identity to the rat ALP sequence and the gene, ASRGL1 (asparaginase-like 1), mapped to chromosome locus 11q12.3. PMID: 11984834
    9. Overexpression of CRASH is associated with metastatic breast cancer. PMID: 19414332
    10. ASRGL1 exhibits beta-aspartyl peptidase activity consistent with plant-type asparaginases. ASRGL1 is shown to be an Ntn hydrolase for which Thr168 serves as the essential N-terminal nucleophile for intramolecular processing and catalysis. PMID: 19839645

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  • 亞細胞定位：
    Cytoplasm. Note=Midpiece of sperm tail.
  • 蛋白家族：
    Ntn-hydrolase family
  • 組織特異性：
    Expressed in brain, kidney, testis and tissues of the gastrointestinal tract. Present in sperm (at protein level). Over-expressed in uterine, mammary, prostatic and ovarian carcinoma.
  • 數據庫鏈接：

    HGNC: 16448

    OMIM: 609212

    KEGG: hsa:80150

    STRING: 9606.ENSP00000301776

    UniGene: Hs.535326