Sheep adiponectin (ADIPOQ)ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊脂聯(lián)素(ADIPOQ)酶聯(lián)免疫試劑盒（CSB-EL001366SH）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的ADIPOQ含量。ADIPOQ即脂聯(lián)素，由脂肪組織分泌。它在能量代謝、胰島素敏感性等方面發(fā)揮重要作用。研究機制上，脂聯(lián)素通過與受體結(jié)合激活下游信號通路，參與調(diào)節(jié)糖脂代謝、抗炎、抗動脈粥樣硬化等，對代謝性疾病防治有潛在價值。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL，該產(chǎn)品適用于綿羊代謝調(diào)控機制研究、疾病模型建立及營養(yǎng)干預(yù)實驗等科研領(lǐng)域，尤其在探索肥胖、糖尿病相關(guān)動物模型中具有重要應(yīng)用價值，為畜牧業(yè)健康監(jiān)測和基礎(chǔ)醫(yī)學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ADIPOQ
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

3.125 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-98
1:200	Average %	103
	Range %	99-106
1:400	Average %	98
	Range %	93-104
1:800	Average %	92
	Range %	87-96

回收率：

The recovery of sheep ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	89	84-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.