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Sheep Serum Amyloid A,SAA ELISA Kit

  • 中文名稱:
    綿羊血清淀粉樣蛋白A(SAA)酶聯免疫試劑盒
  • 貨號:
    CSB-E14973Sh
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    綿羊血清淀粉樣蛋白A(SAA)酶聯免疫試劑盒(CSB-E14973Sh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SAA1含量。試劑盒檢測范圍為1.875 ng/mL-120 ng/mL,特別適用于畜牧科研領域,可用于綿羊炎癥性疾病模型建立、疫苗免疫效果評價、抗炎藥物篩選等基礎研究,亦能為動物病理學研究中組織炎癥程度的定量評估提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    SAA1 ELISA Kit; Serum amyloid A protein ELISA Kit; SAA ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Ovis aries (Sheep)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.875 ng/mL-120 ng/mL
  • 靈敏度:
    0.47 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %94
    Range %90-98
    1:200Average %98
    Range %93-102
    1:400Average %104
    Range %98-108
    1:800Average %89
    Range %84-93
  • 回收率:
    The recovery of sheep SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8682-91
    EDTA plasma (n=4)9490-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    1200.086 0.088 0.087
    600.136 0.142 0.139
    300.247 0.231 0.239
    150.362 0.372 0.367
    7.50.553 0.566 0.560
    3.750.924 0.984 0.954
    1.8751.324 1.401 1.363
    02.448 2.543 2.496
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 相關疾病:
    Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    SAA family
  • 組織特異性:
    Expressed by the liver; secreted in plasma.