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Sheep C-reactive protein(CRP) ELISA Kit

  • 中文名稱:
    綿羊C反應蛋白(CRP)酶聯免疫試劑盒
  • 貨號:
    CSB-E15081Sh
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    綿羊C反應蛋白(CRP)酶聯免疫試劑盒(CSB-E15081Sh)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CRP含量。CRP(C反應蛋白)是一種由肝臟合成的蛋白質,當體內發生炎癥時,CRP水平會升高。它作為炎癥標志物,能結合肺炎鏈球菌等病原體,激活補體系統,增強白細胞吞噬作用,參與非特異性免疫反應。試劑盒檢測范圍為0.625 μg/mL-40 μg/mL,適用于綿羊模型中炎癥機制研究、疾病進程評估及免疫調控相關科研實驗,例如感染性疾病模型構建中CRP動態監測、應激反應評估或藥物干預效果分析本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Ovis aries (Sheep)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    0.625 μg/mL-40 μg/mL
  • 靈敏度:
    0.156 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %96
    Range %92-100
    1:4Average %92
    Range %87-96
    1:8Average %95
    Range %87-98
  • 回收率:
    The recovery of sheep CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-96
    EDTA plasma (n=4)9084-95
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/mlOD1OD2AverageCorrected
    402.473 2.454 2.464 2.376
    202.017 1.998 2.008 1.920
    101.600 1.563 1.582 1.494
    51.091 1.055 1.073 0.985
    2.50.689 0.664 0.677 0.589
    1.250.407 0.399 0.403 0.315
    0.6250.261 0.245 0.253 0.165
    00.089 0.087 0.088  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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