Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

綿羊5羥色胺/血清素/血清胺(5HT/ST)酶聯免疫試劑盒（CSB-E14929Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的5HT/ST含量。5HT即5-羥色胺，是人體內重要神經遞質。5HT/ST靶點相關研究聚焦其在調節情緒、認知等方面作用機制。通過藥物作用于該靶點，可調節5HT水平與功能，用于治療如抑郁癥、焦慮癥等精神疾病，目前仍在不斷深入探索以挖掘更多臨床價值。試劑盒檢測范圍為0.625 ng/mL-10 ng/mL，適用于綿羊模型在神經生物學、藥理學及動物行為學研究中的應用，例如評估應激狀態下神經遞質動態變化、藥物干預對血清素系統的影響或消化道功能相關機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

5HT/ST
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測范圍：

0.625 ng/mL-10 ng/mL
靈敏度：

0.312 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	84
	Range %	80-92
1:40	Average %	97
	Range %	91-105
1:80	Average %	96
	Range %	90-101
1:160	Average %	93
	Range %	86-98

回收率：

The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.