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  4. Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

  • 中文名稱：
    大鼠組織因子與活化凝血因子7復合物酶聯免疫試劑盒
  • 貨號：
    CSB-EQ027252RA
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠組織因子與活化凝血因子7復合物酶聯免疫試劑盒（CSB-EQ027252RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TF含量。TF即組織因子，是一種跨膜糖蛋白。它是外源性凝血途徑的啟動因子，在生理止血中起關鍵作用。研究發現TF還參與腫瘤生長、轉移及血管生成等。其作用機制主要是通過與凝血因子結合，啟動凝血級聯反應并激活相關信號通路。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于心血管疾病、血栓性疾病等動物模型的機制研究，或抗凝藥物、靶向治療方案的體外效果評估。為凝血相關基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 107
    Range % 98-110
    1:2 Average % 103
    Range % 96-107
    1:4 Average % 98
    Range % 89-102
    1:8 Average % 105
    Range % 98-110
  • 回收率：
    The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 85-96
    EDTA plasma (n=4) 102 95-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.708 2.673 2.691 2.589
    500 2.243 2.150 2.197 2.095
    250 1.419 1.469 1.444 1.342
    125 0.702 0.734 0.718 0.616
    62.5 0.412 0.425 0.419 0.317
    31.2 0.251 0.263 0.257 0.155
    15.6 0.167 0.172 0.170 0.068
    0 0.101 0.103 0.102 　
  • 數據處理：
  • 貨期：
    3-5 working days

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