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  4. Rat placenta growth factor,PLGF ELISA kit

Rat placenta growth factor,PLGF ELISA kit

  • 中文名稱：
    大鼠胎盤生長(zhǎng)因子(PLGF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07400r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胎盤生長(zhǎng)因子(PLGF)酶聯(lián)免疫試劑盒（CSB-E07400r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PGF含量。PGF（胎盤生長(zhǎng)因子）是VEGF家族成員之一，主要在胎盤滋養(yǎng)層細(xì)胞中表達(dá)，對(duì)血管生成和胚胎滋養(yǎng)層生長(zhǎng)至關(guān)重要。其研究機(jī)制涉及通過(guò)結(jié)合VEGF受體促進(jìn)血管內(nèi)皮細(xì)胞增殖和遷移，增強(qiáng)血管通透性與穩(wěn)定性。PGF在多種病理過(guò)程中發(fā)揮作用，如心血管疾病和腫瘤生長(zhǎng)，具有廣泛的生物學(xué)意義和研究?jī)r(jià)值。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，支持科研人員開(kāi)展大鼠模型中妊娠并發(fā)癥機(jī)制、組織修復(fù)調(diào)控或血管生成相關(guān)疾病研究。兼容多種生物樣本類型，特別適用于探究胎盤發(fā)育異常、心血管疾病模型或腫瘤微環(huán)境中的PLGF動(dòng)態(tài)變化，為體外實(shí)驗(yàn)提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Pgf ELISA Kit; Plgf ELISA Kit; Placenta growth factor ELISA Kit; PlGF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-95  
    1:2 Average % 94  
    Range % 89-98  
    1:4 Average % 93  
    Range % 87-97  
    1:8 Average % 99  
    Range % 93-102  
  • 回收率：
    The recovery of rat PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 94 91-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    200 2.246 2.234 2.240 2.085  
    100 1.472 1.456 1.464 1.309  
    50 0.901 0.914 0.908 0.753  
    25 0.639 0.649 0.644 0.489  
    12.5 0.364 0.389 0.377 0.222  
    6.25 0.300 0.308 0.304 0.149  
    3.12 0.252 0.242 0.247 0.092  
    0 0.156 0.154 0.155 　  
  • 本試劑盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat PLGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled PLGF antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth.
  • 基因功能參考文獻(xiàn)：
    1. Studied the influence of dendrobium nobile Lindley (DNL) extract and its bibenzyl component moscatilin on the expression of placental growth factor (PLGF) and Norrie disease protein (NDP) in hypoxia model in retinal cells. Found DNL and moscatilin are able to protect against retinal ischemic/hypoxic changes respectively by downregulating PLGF and upregulating NDP. PMID: 29933759
    2. this paper show that knockdown of PLGF mitigates damage in hyperoxic lung by blocking of NFkappaB signaling PMID: 27280587
    3. PLGF triggers focal adhesion disassembly in alveolar type II cells via inhibiting the activation of focal adhesion kinase. PMID: 25515701
    4. PlGF had a positive feedback regulation with 15-lipoxygenase expression and 15-hydroxyeicosatetraenoic acid generation. PMID: 24001991
    5. placental gene expression is modulated by gestational conditions that might disrupt the fetal growth PMID: 23691181
    6. PlGF repairs myocardial ischemia through mechanisms of angiogenesis, cardioprotection and recruitment of myo-angiogenic competent marrow progenitors PMID: 21969865
    7. augmentation of brain Placental growth factor is associated with development of epilepsy. PMID: 22079325
    8. PLGF expression in the lung tissue in rats with paraquat-induced pulmonary fibrosis is upregulated. PMID: 17997886
    9. This is the first demonstration that sustained intraocular PGF production induces vascular and retinal changes similar to those observed in the early stages of diabetic retinopathy. PMID: 21408222
    10. Intramyocardial injections of autologous myoblasts overexpressing placental growth factor improved cardiac function, attenuated adverse cardiac remodeling, induced angiogenesis, and probably enhanced survival of grafted myoblasts. PMID: 21329947
    11. This study shows for the first time the placental growth factor upregulation in regenerating livers, which is related to hypoxia stimulation and liver growth. PMID: 21088600
    12. Endothelial cell-released H2O2 acts as a positive regulator of placenta growth factor gene and protein expression in vascular smooth muscle cells. PMID: 21123739
    13. The ARPE19 human junctions in culture are modulated by VEGF through VEGFR-1 but not through VEGFR-2. PMID: 18421240
    14. The levels of PlGF-2 in non-malformed embryos of diabetic gestation and their placentas were significantly (P < 0.05) lower than the average of controls. PMID: 19048427

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: rno:94203

    STRING: 10116.ENSRNOP00000007790

    UniGene: Rn.6960