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Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

  • 中文名稱:
    大鼠核因子-κB亞基p65親和肽(NF-κB p65)酶聯免疫試劑盒
  • 貨號:
    CSB-E08788r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠核因子-κB亞基p65親和肽(NF-κB p65)酶聯免疫試劑盒(CSB-E08788r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的NFκB p65含量。NFκB p65是重要的轉錄因子,在細胞的炎癥、免疫、增殖和凋亡等多種生理病理過程中發揮關鍵作用。其激活機制多樣,與多條信號通路相互關聯。對其研究有助于深入了解疾病發病機制,為開發針對性療法如抗炎、抗腫瘤藥物等提供理論基礎。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,適用于基礎科研中炎癥機制研究、免疫調控實驗或藥物干預效果評估,例如在動物模型中分析NF-κB信號通路激活狀態,或探究氧化應激、感染性疾病等實驗條件下p65亞基的動態表達。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    NF-Kappa B,nuclear factor-kappa B
  • 縮寫:
    NFκB p65
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    0.39 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %89
    Range %86-92
    1:200Average %99
    Range %97-103
    1:400Average %89
    Range %85-93
    1:800Average %95
    Range %91-99
  • 回收率:
    The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9490-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.039 2.022 2.031 1.887
    501.334 1.326 1.330 1.186
    250.908 0.902 0.905 0.761
    12.50.566 0.557 0.562 0.418
    6.250.358 0.364 0.361 0.217
    3.120.291 0.281 0.286 0.142
    1.560.243 0.232 0.238 0.094
    00.145 0.143 0.144  
  • 數據處理:
  • 貨期:
    3-5 working days

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