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  4. Rat lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

Rat lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

  • 中文名稱：
    大鼠脂蛋白磷脂酶A2(Lp-PLA2)酶聯免疫試劑盒
  • 貨號：
    CSB-E08320r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠脂蛋白磷脂酶A2(Lp-PLA2)酶聯免疫試劑盒（CSB-E08320r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Lp-PLA2含量。Lp-PLA2（脂蛋白相關磷脂酶A2）是一種與動脈粥樣硬化和心血管疾病密切相關的標志物，由血管內膜中的巨噬細胞、T細胞和肥大細胞分泌。其水平高低與腦卒中和心血管疾病風險密切相關，通過水解氧化低密度脂蛋白中的氧化磷脂，生成脂類促炎物質，促進動脈粥樣硬化。試劑盒檢測范圍為0.78 IU/mL-50 IU/mL，該產品適用于科研領域中對大鼠模型血清或血漿樣本的Lp-PLA2水平監測，例如心血管疾病動物模型的病理機制研究、藥物干預效果評估或炎癥相關代謝調控實驗，為體外實驗提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    Lp-PLA2
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.78 IU/mL-50 IU/mL
  • 靈敏度：
    0.195 IU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 88-96    
    1:2 Average % 105    
    Range % 100-110    
    1:4 Average % 86    
    Range % 82-90    
    1:8 Average % 93    
    Range % 89-98    
  • 回收率：
    The recovery of rat Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 88 84-92    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    IU/ml OD1 OD2 Average Corrected    
    50 2.203 2.361 2.282 2.152    
    25 1.924 2.040 1.982 1.852    
    12.5 1.512 1.459 1.486 1.356    
    6.25 0.936 0.902 0.919 0.789    
    3.12 0.477 0.485 0.481 0.351    
    1.56 0.300 0.318 0.309 0.179    
    0.78 0.241 0.234 0.238 0.108    
    0 0.132 0.127 0.130 　    
  • 數據處理：
  • 貨期：
    3-5 working days

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