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  4. Rat heat shock protein,HSP47 ELISA Kit

Rat heat shock protein,HSP47 ELISA Kit

  • 中文名稱：
    大鼠熱休克蛋白47(HSP47)酶聯免疫試劑盒
  • 貨號：
    CSB-E11328r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠熱休克蛋白47(HSP47)酶聯免疫試劑盒（CSB-E11328r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SERPINH1含量。SERPINH1是一個具有重要研究價值的靶點。它在細胞內參與蛋白質折疊、穩定等過程。目前研究聚焦其在疾病發生發展中的機制，如腫瘤進展、纖維化等病理進程，解析其作用機制或能為開發相關治療藥物、改善治療方案提供新方向。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于科研領域中對大鼠模型膠原代謝相關機制的研究，例如肝/肺纖維化疾病模型的分子機制探索、皮膚或骨骼損傷修復過程中膠原沉積的動態分析，以及藥物干預下HSP47表達水平變化的評估。試劑盒兼容多類型生物樣本，可為體外實驗或動物模型研究提供高靈敏度的檢測方案，助力科研人員高效獲取HSP47在膠原相關通路中的調控數據。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Serpinh1 ELISA Kit; Cbp1 ELISA Kit; Hsp47 ELISA Kit; Serpin H1 ELISA Kit; 47 kDa heat shock protein ELISA Kit; Collagen-binding protein ELISA Kit; Colligin ELISA Kit; GP46 ELISA Kit
  • 縮寫：
    SERPINH1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %81-93
    1:2Average %102
    Range %98-105
    1:4Average %92
    Range %88-96
    1:8Average %95
    Range %91-99
  • 回收率：
    The recovery of rat HSP47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-96
    EDTA plasma (n=4)8783-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.311 2.507 2.409 2.261
    51.740 1.645 1.693 1.545
    2.51.087 1.124 1.106 0.958
    1.250.664 0.652 0.658 0.510
    0.6250.420 0.433 0.427 0.279
    0.3120.339 0.327 0.333 0.185
    0.1560.226 0.230 0.228 0.080
    00.147 0.148 0.148
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 基因功能參考文獻：
    1. The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production. PMID: 25526199
    2. miR-29b down-regulates heat shock protein 47 and LOX expression. PMID: 24650661
    3. HSP47 specifically modulates TGF-beta1-induced extracellular matrix protein synthesis in HK-2 cells PMID: 22718885
    4. Heat shock protein 47 (HSP47) antisense oligonucleotides reduce cardiac remodeling and improve cardiac function in a rat model of myocardial infarction. PMID: 21412710
    5. Involvement of HSP47 in progression of peritoneal fibrosis. Inhibition of HSP47 expression might merit further clinical investigation for treatment of peritoneal fibrosis in continuous ambulatory peritoneal dialysis. PMID: 12911538
    6. Microgravity differentially modulated the expression of the molecular chaperone HSP47 in osteoblasts PMID: 15033773
    7. expression of HSP47, a collagen-specific molecular chaperone, responds to gravitational changes, including microgravity and hypergravity in vitro and in vivo PMID: 17054723

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  • 亞細胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Serpin family
  • 數據庫鏈接：