Rat fibroblast growth factor 21 (FGF21) ELISA kit

產品詳情

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文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠成纖維細胞生長因子21(FGF21)酶聯免疫試劑盒（CSB-EL008627RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF21含量。FGF21是一種成纖維細胞生長因子。它在糖脂代謝等生理過程中發揮重要作用，具有改善胰島素敏感性、降低血糖和血脂的潛在功效。研究機制主要圍繞其與受體結合，激活下游信號通路，調節細胞代謝活動，為代謝性疾病治療帶來新方向。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，該產品適用于代謝性疾病基礎研究領域，包括肥胖、糖尿病模型中的FGF21動態變化分析，或肝臟代謝調控機制的探索。為研究者開展能量代謝相關分子機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

FGF21
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	106
	Range %	102-114
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rat FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	96	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.