Rat eosinophil cationic protein,ECP ELISA Kit

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產品詳情

產品描述：

大鼠嗜酸性粒細胞陽離子蛋白(ECP)酶聯免疫試劑盒（CSB-E11238r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的RNASE3含量。RNASE3是一種重要靶點。它在生物體內具有多種生理功能，參與免疫防御等過程。相關研究集中在其作用機制，比如與病原體的相互作用，以及在炎癥反應中扮演的角色等，深入探究有助于為相關疾病的治療提供新思路。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于大鼠模型中的炎癥機制研究、藥效評估或疾病生物標志物探索，例如呼吸道炎癥模型的病理進程監測、抗過敏藥物干預效果分析等場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Rnase3 ELISA Kit; Ear11 ELISA Kit; Rns3 ELISA Kit; Eosinophil cationic protein ELISA Kit; ECP ELISA Kit; EC 3.1.27.- ELISA Kit; Eosinophil secondary granule ribonuclease 11 ELISA Kit; EAR-11 ELISA Kit; Ribonuclease 3 ELISA Kit; RNase 3 ELISA Kit
縮寫：

RNASE3
Uniprot No.：

P70709
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	101
	Range %	96-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	82-91

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities.
亞細胞定位：

Cytoplasmic granule.
蛋白家族：

Pancreatic ribonuclease family
數據庫鏈接：

KEGG: rno:100361866

STRING: 10116.ENSRNOP00000066375

UniGene: Rn.10626