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Rat apolipoprotein B-48 (ApoB48) ELISA kit

  • 中文名稱:
    大鼠載脂蛋白B-48(ApoB48)酶聯免疫試劑盒
  • 貨號:
    CSB-EQ027557RA
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠載脂蛋白B-48(ApoB48)酶聯免疫試劑盒(CSB-EQ027557RA)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的ApoB48含量。ApoB48 是一種重要靶點。它主要在小腸合成,參與乳糜微粒組裝和分泌。研究發現其與脂質代謝密切相關,異常升高可能導致血脂異常等問題。目前科研聚焦于通過抑制 ApoB48 生成或功能,調節脂質代謝,為治療相關疾病提供新思路。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL,適用于營養干預實驗、代謝性疾病模型研究、藥物對腸道脂質吸收影響的評估等科研領域,為探索脂代謝調控機制及心血管疾病相關研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    ApoB48
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    0.312 μg/mL-20 μg/mL
  • 靈敏度:
    0.078 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ApoB48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 88  
    Range % 84-92  
    1:200 Average % 96  
    Range % 92-102  
    1:400 Average % 95  
    Range % 89-100  
    1:800 Average % 92  
    Range % 89-96  
  • 回收率:
    The recovery of rat ApoB48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-102  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml. OD1 OD2 Average    
    20 0.074 0.076 0.075    
    10 0.120 0.126 0.123    
    5 0.182 0.185 0.184    
    2.5 0.283 0.286 0.285    
    1.25 0.451 0.468 0.460    
    0.625 0.770 0.785 0.778    
    0.312 1.122 1.162 1.142    
    0 2.371 2.392 2.382    
  • 數據處理:
  • 貨期:
    3-5 working days

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