Rat alcohol dehydrogenase,ADH ELISA Kit

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Rat alcohol dehydrogenase,ADH ELISA Kit

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中文名稱：

大鼠乙醇脫氫酶(ADH)酶聯免疫試劑盒
貨號：

CSB-E11180r
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

大鼠乙醇脫氫酶(ADH)酶聯免疫試劑盒（CSB-E11180r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADH含量。ADH即抗利尿激素，它主要由下丘腦視上核和室旁核的神經內分泌細胞合成。其作用機制主要是增加腎小管對水的重吸收，以維持機體水平衡。在臨床上，ADH水平異常會引發如尿崩癥等疾病，相關研究有助于深入了解水代謝調節機制與疾病治療。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于體外研究乙醇代謝調控機制、評估肝損傷模型中ADH表達水平動態變化，或探究藥物干預對酶活性影響的實驗場景；為研究乙醇代謝通路、肝臟功能評估及毒性物質作用機制提供高效的科研工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Adh1 ELISA Kit; Adh-1 ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; EC 1.1.1.1 ELISA Kit; Alcohol dehydrogenase A subunit ELISA Kit
縮寫：

ADH
Uniprot No.：

P06757
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-95
1:2	Average %	101
	Range %	96-105
1:4	Average %	93
	Range %	88-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	88	80-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.312	2.456	2.384	2.237
10	1.586	1.712	1.649	1.502
5	1.009	1.054	1.032	0.885
2.5	0.641	0.632	0.637	0.490
1.25	0.412	0.435	0.424	0.277
0.625	0.287	0.274	0.281	0.134
0.312	0.211	0.218	0.215	0.068
0	0.146	0.147	0.147

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

基因功能參考文獻：
1. study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism. PMID: 22214999
2. rat hepatic Class I ADH gene expression is regulated in vivo by ethanol in a mechanism involving the C/EBP site and the enhancer binding proteins beta and gamma PMID: 12213809
3. Results describe the expression patterns of alcohol dehydrogenases I, III, and IV in adult rat, mouse and human tissues using radioactive oligonucleotide in situ hybridization. PMID: 12631290
4. activity and expression in gastrointestinal tissue homogenates PMID: 12787032
5. class I alcohol dehydrogenase is induced by sterol regulatory element-binding protein-1 in rat liver PMID: 15123720
6. Alcohol dehydrogenase PMID: 16109828
7. Cellular expression of Adh1 in the GI tract was studied. PMID: 17257171
8. A high ADH activity leads to low ethanol intake in animals. PMID: 17488809
9. ADH class I gene is progesterone-responsive in the uterus. PMID: 18219182
10. Results revealed that kidney alcohol dehydrogenase activities increased significantly after ethanol administration, but the electrophoretic pattern of alcohol dehydrogenase isoforms was unmodified. PMID: 16421892
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亞細胞定位：

Cytoplasm.
蛋白家族：

Zinc-containing alcohol dehydrogenase family, Class-I subfamily
數據庫鏈接：

KEGG: rno:24172

STRING: 10116.ENSRNOP00000016346

UniGene: Rn.40222

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.