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  4. Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

  • 中文名稱：
    大鼠內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制劑(vaspin)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09813r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制劑(vaspin)酶聯(lián)免疫試劑盒（CSB-E09813r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPINA12含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，可廣泛應(yīng)用于肥胖動物模型建立、代謝性疾病機制研究、脂肪組織功能評估以及藥物干預(yù)效果評價等科研領(lǐng)域，為探索vaspin在能量代謝調(diào)控中的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Serpina12 ELISA Kit; Serpin A12 ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Vaspin ELISA Kit; Visceral adipose-specific serpin ELISA Kit
  • 縮寫：
    SERPINA12
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %94
    Range %90-98
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %92-104
  • 回收率：
    The recovery of rat vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 105100-110
    EDTA plasma (n=4)9590-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.679 2.576 2.628 2.479
    10002.071 2.114 2.093 1.944
    5001.582 1.472 1.527 1.378
    2500.958 0.913 0.936 0.787
    1250.580 0.589 0.585 0.436
    62.50.412 0.407 0.410 0.261
    31.250.302 0.279 0.291 0.142
    00.147 0.151 0.149
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 組織特異性：
    Expressed in visceral adipose tissues.
  • 數(shù)據(jù)庫鏈接：