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  4. Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

  • 中文名稱：
    大鼠血管活性腸肽(VIP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08355r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠血管活性腸肽(VIP)酶聯(lián)免疫試劑盒（CSB-E08355r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VIP含量。VIP即血管活性腸肽，具有廣泛生物學(xué)活性。它參與調(diào)節(jié)心血管、消化等系統(tǒng)功能。在研究機(jī)制上，VIP通過與特定受體結(jié)合，激活細(xì)胞內(nèi)信號(hào)通路，影響多種生理和病理過程，在神經(jīng)保護(hù)、抗炎等方面展現(xiàn)潛在治療價(jià)值。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于神經(jīng)生物學(xué)、消化系統(tǒng)疾病模型研究，可分析大鼠外周循環(huán)或組織局部VIP表達(dá)水平，為探究胃腸動(dòng)力障礙、炎癥調(diào)節(jié)機(jī)制及神經(jīng) - 免疫交互作用提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %97
    Range %93-101
    1:4Average %100
    Range %97-103
    1:8Average %86
    Range %82-92
  • 回收率：
    The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9086-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.384 2.506 2.445 2.324
    501.732 1.813 1.773 1.652
    251.112 1.100 1.106 0.985
    12.50.615 0.626 0.621 0.500
    6.250.386 0.397 0.392 0.271
    3.120.297 0.286 0.292 0.171
    1.560.196 0.193 0.195 0.074
    00.121 0.120 0.121 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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