Rat Thromboxane B2,TXB2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (9) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血栓素B2(TXB2)酶聯(lián)免疫試劑盒（CSB-E08047r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TXB2含量。TXB2是一種由TXA2酶促代謝產(chǎn)生的穩(wěn)定水解產(chǎn)物，是TXA2的穩(wěn)定代謝物，可以反映TXA2的全身生物合成速率。TXB2在生理和病理過(guò)程中扮演重要角色，如參與止血、動(dòng)脈粥樣硬化、血栓形成等。研究TXB2有助于了解相關(guān)疾病的發(fā)生機(jī)制，為疾病診斷和治療提供新的靶點(diǎn)。試劑盒檢測(cè)范圍為3.9 pg/mL-250 pg/mL，適用于心血管疾病動(dòng)物模型研究、抗血小板藥物藥效評(píng)估、炎癥相關(guān)機(jī)制探索等科研領(lǐng)域，滿足基礎(chǔ)研究中對(duì)TXB2動(dòng)態(tài)變化的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

TXB2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.9 pg/mL-250 pg/mL
靈敏度：

0.97 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	95
	Range %	91-102
1:4	Average %	99
	Range %	94-106
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-103
EDTA plasma (n=4)	92	88-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.