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Rat Platelet-derived growth factor DD(PDGF-DD) ELISA kit

  • 中文名稱:
    大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯免疫試劑盒
  • 貨號:
    CSB-EL017711RA
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯免疫試劑盒(CSB-EL017711RA)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PDGF-DD含量。PDGF - DD是一種血小板衍生生長因子異構體。它在細胞的生長、增殖和遷移等過程中發揮重要作用。其研究機制主要聚焦于與受體結合后激活下游信號通路,影響細胞行為,在腫瘤、纖維化等疾病的發生發展中可能有潛在影響,是藥物研發的潛在靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于科研領域探究PDGF-DD在大鼠心血管疾病模型、器官纖維化機制、創傷修復實驗中的動態表達特征,通過血清或血漿樣本可系統性評估循環系統中PDGF-DD水平,而組織勻漿檢測則有助于解析特定器官或病變部位的局部蛋白表達模式,為相關分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    PDGF-DD
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %86
    Range %80-90
    1:2Average %89
    Range %85-96
    1:4Average %90
    Range %84-96
    1:8Average %92
    Range %86-98
  • 回收率:
    The recovery of rat PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-103
    EDTA plasma (n=4)9590-101
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.744 2.678 2.711 2.531
    52.139 2.112 2.126 1.946
    2.51.509 1.476 1.493 1.313
    1.251.025 1.003 1.014 0.834
    0.6250.662 0.651 0.657 0.477
    0.3120.403 0.386 0.395 0.215
    0.1560.292 0.272 0.282 0.102
    00.181 0.179 0.180  
  • 數據處理:
  • 貨期:
    3-5 working days

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