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  4. Rat Platelet-derived growth factor CC(PDGF-CC) ELISA kit

Rat Platelet-derived growth factor CC(PDGF-CC) ELISA kit

  • 中文名稱：
    大鼠血小板衍生生長因子CC(PDGF-CC) 酶聯免疫試劑盒
  • 貨號：
    CSB-EL017710RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠血小板衍生生長因子CC(PDGF-CC) 酶聯免疫試劑盒（CSB-EL017710RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-CC含量。PDGF - CC即血小板衍生生長因子CC，是PDGF家族成員。它在胚胎發育、組織修復等過程有重要作用。其異常表達與腫瘤、纖維化等疾病相關。研究機制多圍繞其與受體結合，激活下游信號通路，影響細胞增殖、遷移等方面。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領域對大鼠模型中PDGF-CC蛋白表達的定量分析，例如在心血管疾病、組織再生或腫瘤機制研究中評估目標分子動態變化本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    PDGF-CC
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 85-95
    1:2 Average % 103
    Range % 99-112
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 90
    Range % 85-94
  • 回收率：
    The recovery of rat PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 82-93
    EDTA plasma (n=4) 95 90-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.143 2.003 2.073 1.893
    500 1.487 1.405 1.446 1.266
    250 0.899 0.927 0.913 0.733
    125 0.603 0.569 0.586 0.406
    62.5 0.391 0.412 0.402 0.222
    31.2 0.270 0.284 0.277 0.097
    15.6 0.240 0.231 0.236 0.056
    0 0.183 0.176 0.180 　
  • 數據處理：
  • 貨期：
    3-5 working days

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