Rat Platelet Factor 4,PF-4 ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠血小板因子4(PF-4/CXCL4)酶聯免疫試劑盒（CSB-E07885r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PF4含量。PF4即血小板因子4，是血小板α顆粒分泌的一種小分子蛋白。在生理上它能趨化炎癥細胞、參與凝血等。研究機制方面，其在血栓形成、腫瘤微環境調節等過程有作用，深入研究它對相關疾病治療靶點的開發具有重要意義。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，靈敏度為0.039 μg/mL?？蒲腥藛T可將其應用于血小板活化機制、血栓相關疾病模型或炎癥微環境等研究領域，為探索PF - 4/CXCL4在病理生理過程中的調控作用提供可靠工具。試劑盒兼容多種生物樣本類型，滿足實驗室基礎研究及藥物開發中的靶點驗證需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
縮寫：

PF4
Uniprot No.：

P06765
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-97
1:2	Average %	95
	Range %	88-99
1:4	Average %	95
	Range %	91-102
1:8	Average %	95
	Range %	87-104

回收率：

The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-99
EDTA plasma (n=4)	97	89-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
基因功能參考文獻：
1. identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells PMID: 28053995
2. A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells. PMID: 12383857
3. this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME PMID: 12609849
4. PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression PMID: 15187018
5. The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05). PMID: 16797058
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亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數據庫鏈接：

KEGG: rno:360918

STRING: 10116.ENSRNOP00000032276

UniGene: Rn.6800