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  4. Rat Pancreatic prohormone(PPY) ELISA kit

Rat Pancreatic prohormone(PPY) ELISA kit

  • 中文名稱：
    大鼠胰多肽酶聯免疫試劑盒
  • 貨號：
    CSB-E12747r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠胰多肽酶聯免疫試劑盒（CSB-E12747r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PPY含量。PPY即多肽YY，是由腸道L細胞分泌的一種激素。它能調節食欲、能量代謝等。研究機制上，其可與特定受體結合，作用于下丘腦等腦區以抑制食欲；也能影響胃腸道運動和消化液分泌，在維持機體能量穩態等生理過程中發揮重要作用。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，適用于科研場景中探究胰多肽在消化系統疾病模型、代謝調控機制或藥物干預實驗中的動態變化，為動物實驗提供可靠的內分泌指標檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    PPY
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.2 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 104    
    Range % 93-109    
    1:200 Average % 100    
    Range % 91-104    
    1:400 Average % 97    
    Range % 88-101    
    1:800 Average % 90    
    Range % 82-94    
  • 回收率：
    The recovery of rat PPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 83-94    
    EDTA plasma (n=4) 100 91-104    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    50 2.069 1.932 2.001 1.899    
    25 1.429 1.466 1.448 1.346    
    12.5 0.946 0.917 0.932 0.830    
    6.25 0.662 0.651 0.657 0.555    
    3.12 0.348 0.340 0.344 0.242    
    1.56 0.242 0.237 0.240 0.138    
    0.78 0.164 0.159 0.162 0.060    
    0 0.104 0.100 0.102 　    
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    NPY family
  • 數據庫鏈接：

    KEGG: rno:24677

    STRING: 10116.ENSRNOP00000028318

    UniGene: Rn.9755