Rat Motilin,MTL ELISA Kit

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中文名稱：

大鼠胃動(dòng)素(MTL)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08208r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠胃動(dòng)素(MTL)酶聯(lián)免疫試劑盒（CSB-E08208r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MTL含量。大鼠胃動(dòng)素（MTL）是一種由內(nèi)分泌Mo細(xì)胞分泌的22個(gè)氨基酸組成的活性肽，主要在消化間期周期性釋放，促進(jìn)胃腸運(yùn)動(dòng)和胃蛋白酶分泌。其作用機(jī)制涉及與胃腸平滑肌上的胃動(dòng)素受體結(jié)合，增強(qiáng)平滑肌收縮，加速胃排空，并調(diào)節(jié)消化間期胃腸活動(dòng)，如移行性復(fù)合運(yùn)動(dòng)（MMC）。MTL的研究對(duì)于理解胃腸動(dòng)力和消化功能具有重要意義。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于胃腸動(dòng)力障礙動(dòng)物模型研究、藥物干預(yù)后胃動(dòng)素分泌評(píng)估，以及消化道組織樣本中MTL表達(dá)的動(dòng)態(tài)監(jiān)測(cè)，為胃腸生理學(xué)、病理學(xué)機(jī)制研究及藥效學(xué)評(píng)價(jià)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

N/A
縮寫：

MTL
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-96
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	96-106
1:8	Average %	95
	Range %	89-102

回收率：

The recovery of rat MTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-108
EDTA plasma (n=4)	96	92-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.725	2.905	2.815	2.614
1000	2.222	2.102	2.162	1.961
500	1.527	1.507	1.517	1.316
250	0.920	0.930	0.925	0.724
125	0.577	0.589	0.583	0.382
62.5	0.432	0.423	0.428	0.227
31.25	0.302	0.296	0.299	0.098
0	0.197	0.204	0.201