Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠低密度脂蛋白膽固醇(LDL-C)酶聯免疫試劑盒（CSB-E16561r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDL-C含量。LDL-C（低密度脂蛋白膽固醇）作為降脂治療的重要靶點，其研究背景源于其對心血管疾病風險的預測作用。研究機制主要圍繞LDL-C在動脈粥樣硬化形成中的作用，通過降低LDL-C水平來預防心血管疾病。試劑盒檢測范圍為125 ng/mL-8000 ng/mL，適用于實驗室開展動脈粥樣硬化模型評估、脂質代謝通路研究、降脂藥物篩選等科研項目，為心血管疾病基礎研究及營養學實驗提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

LDL-C
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 ng/mL-8000 ng/mL
靈敏度：

62.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	94-102
1:200	Average %	94
	Range %	91-97
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	84-92

回收率：

The recovery of rat LDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-99
EDTA plasma (n=4)	103	98-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.