Rat Leukotriene B4,LT-B4 ELISA Kit

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中文名稱(chēng)：

大鼠白三烯B4(LTB4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08035r
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
促銷(xiāo)：
其他：

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠白三烯B4(LTB4)酶聯(lián)免疫試劑盒（CSB-E08035r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LT-B4含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于實(shí)驗(yàn)室環(huán)境中開(kāi)展炎癥相關(guān)疾病模型研究、藥物干預(yù)效果評(píng)估或免疫調(diào)控機(jī)制探索等科研場(chǎng)景，尤其適合同步分析血液樣本與組織局部炎癥介質(zhì)的表達(dá)水平，為深入解析LTB4在病理生理過(guò)程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫(xiě)：

LT-B4
種屬：

Rattus norvegicus (Rat)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

89-99

1:2

Average %

Range %

91-103

1:4

Average %

Range %

92-104

1:8

Average %

Range %

88-97

回收率：

The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	97	93-106

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.442	2.541	2.492	2.320
500	1.887	1.986	1.937	1.765
250	1.282	1.263	1.273	1.101
125	0.830	0.821	0.826	0.654
62.5	0.511	0.502	0.507	0.335
31.2	0.370	0.361	0.366	0.194
15.6	0.264	0.259	0.262	0.090
0	0.171	0.172	0.172