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Rat Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit

  • 中文名稱:
    大鼠細胞角蛋白18片段Asp396(K18-Asp396)酶聯免疫試劑盒
  • 貨號:
    CSB-E13708r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠細胞角蛋白18片段Asp396(K18-Asp396)酶聯免疫試劑盒(CSB-E13708r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CK 18M30含量。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL,適用于基礎科研中動態監測細胞凋亡水平、評估藥物干預效果或探究疾病模型中上皮細胞損傷機制。適用于肝臟疾病模型研究、腫瘤生物學實驗或毒理學評估等非臨床科研場景,為細胞死亡相關分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    CK 18M30
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 mIU/mL-100 mIU/mL
  • 靈敏度:
    0.39 mIU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 91-102  
    1:2 Average % 94  
    Range % 90-98  
    1:4 Average % 84  
    Range % 80-88  
    1:8 Average % 103  
    Range % 99-112  
  • 回收率:
    The recovery of rat CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-94  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    100 2.599 2.512 2.556 2.407  
    50 1.769 1.721 1.745 1.596  
    25 1.066 1.024 1.045 0.896  
    12.5 0.607 0.655 0.631 0.482  
    6.25 0.358 0.389 0.374 0.225  
    3.12 0.279 0.295 0.287 0.138  
    1.56 0.213 0.209 0.211 0.062  
    0 0.154 0.143 0.149    
  • 數據處理:
  • 貨期:
    3-5 working days

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