Rat Ischemia Modified Albumin,IMA ELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠缺血修飾白蛋白(IMA)酶聯免疫試劑盒（CSB-E13620r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IMA含量。大鼠缺血修飾白蛋白（IMA）是心肌缺血早期標志物，其形成與缺血時白蛋白與金屬離子結合能力改變有關。IMA檢測可用于急性冠脈綜合征（ACS）的早期診斷和危險分層，對心肌缺血再灌注治療有指導意義。試劑盒檢測范圍為0.9 U/mL-60 U/mL，適用于大鼠心血管疾病機制研究、缺血再灌注損傷模型評估、藥物干預效果分析等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IMA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.9 U/mL-60 U/mL
靈敏度：

0.225 U/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-95
1:2	Average %	96
	Range %	90-103
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	82-92

回收率：

The recovery of rat IMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	89	85-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.