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  4. Rat Heat shock protein HSP 90-alpha (Hsp90aa1/Hsp86/Hspca) ELISA kit

Rat Heat shock protein HSP 90-alpha (Hsp90aa1/Hsp86/Hspca) ELISA kit

  • 中文名稱：
    大鼠熱休克蛋白90-alpha(Hsp90aa1/Hsp86/Hspca)酶聯免疫試劑盒
  • 貨號：
    CSB-E08309r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠熱休克蛋白90-alpha(Hsp90aa1/Hsp86/Hspca)酶聯免疫試劑盒（CSB-E08309r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Hsp90aa1含量。Hsp90aa1 是一種重要靶點。它在細胞應激反應、蛋白折疊等方面發揮關鍵作用。研究聚焦其與腫瘤發生發展關聯，通過抑制它的活性，干擾腫瘤細胞的信號通路、影響蛋白穩定性，為開發新型抗癌藥物提供了理論基礎和潛在方向。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研實驗中評估大鼠模型在熱應激、氧化損傷或疾病狀態下Hsp90aa1的動態變化，為探究其分子調控機制、篩選潛在調控藥物提供可靠工具；支持研究者高效開展心血管疾病、代謝綜合征等基礎醫學領域的分子機制研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Hsp90aa1 ELISA Kit; Hsp86 ELISA Kit; HspcaHeat shock protein HSP 90-alpha ELISA Kit; Heat shock 86 kDa ELISA Kit; HSP 86 ELISA Kit; HSP86 ELISA Kit
  • 縮寫：
    HSP-90
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hsp90aa1/Hsp86/Hspca in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-97
    1:2Average %96
    Range %89-103
    1:4Average %102
    Range %98-106
    1:8Average %87
    Range %81-95
  • 回收率：
    The recovery of rat Hsp90aa1/Hsp86/Hspca spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-94
    EDTA plasma (n=4)10197-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.517 2.735 2.626 2.433
    101.772 1.859 1.816 1.623
    51.159 1.164 1.162 0.969
    2.50.680 0.740 0.710 0.517
    1.250.517 0.539 0.528 0.335
    0.6250.374 0.388 0.381 0.188
    0.3120.290 0.314 0.302 0.109
    00.189 0.197 0.193
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochodria outer membrane which promotes host antiviral response.
  • 亞細胞定位：
    Nucleus. Cytoplasm. Melanosome. Cell membrane. Mitochondrion.
  • 蛋白家族：
    Heat shock protein 90 family
  • 數據庫鏈接：