Rat Endostatin,ES ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠內皮抑素(ES)酶聯免疫試劑盒（CSB-E07975r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ES含量。大鼠內皮抑素（ES）是一種血管生成抑制因子，通過抑制內皮細胞增殖和遷移，發揮抗血管生成作用，促進腫瘤細胞凋亡。其研究機制涉及抑制VEGF表達、抑制新生血管生成，并可能通過血管“正常化”作用提高放療敏感性。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，在科研領域，本產品可廣泛應用于癌癥模型血管抑制效應評估、心血管疾病機制研究、抗血管生成藥物開發等基礎研究場景，為探究ES在病理生理過程中的動態變化及其調控網絡提供標準化工具，助力血管生物學、腫瘤學等領域的基礎科研工作。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP
縮寫：

ES
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	101
	Range %	97-104
1:4	Average %	90
	Range %	87-94
1:8	Average %	100
	Range %	95-103

回收率：

The recovery of rat ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-98
EDTA plasma (n=4)	95	92-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.