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  4. Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

  • 中文名稱：
    大鼠肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒
  • 貨號：
    CSB-E14403r
  • 樣本類型：
    serum, urine, tissue homogenates, plasma
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒（CSB-E14403r）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織勻漿、血漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。測定其在血液中的含量，能反映心肌損傷情況，是診斷急性心肌梗死的重要指標。研究機制主要是在心肌受損時，CK-MB釋放入血，通過檢測血中濃度變化輔助病情判斷。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，廣泛應用于心血管疾病動物模型研究，如心肌損傷機制探索、心臟保護藥物篩選等科研領域。適用于心肌損傷動態監測、藥物干預效果評估等實驗需求。為心血管相關基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    CK-MB
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 98-103  
    1:2 Average % 96  
    Range % 92-100  
    1:4 Average % 92  
    Range % 87-98  
    1:8 Average % 89  
    Range % 84-94  
  • 回收率：
    The recovery of rat CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 96-104  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.646 2.687 2.667 2.564  
    10 1.834 1.856 1.845 1.742  
    5 1.096 1.099 1.098 0.995  
    2.5 0.593 0.583 0.588 0.485  
    1.25 0.356 0.368 0.362 0.259  
    0.625 0.229 0.249 0.239 0.136  
    0.312 0.165 0.174 0.170 0.067  
    0 0.101 0.105 0.103 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CK-MB antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled CK-MB antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

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  • 最新研究進展：
    CK-MB是一種心肌特異性蛋白質，是臨床上常用的心肌損傷指標之一。CK-MB可以用于診斷急性心肌梗死等心血管疾病。近年來，研究人員發現CK-MB在許多其他生理和病理過程中也發揮重要作用，如運動訓練、重癥肌無力等。