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Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit

  • 中文名稱:
    大鼠Ⅱ型膠原(Col Ⅱ)酶聯免疫試劑盒
  • 貨號:
    CSB-E08092r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠Ⅱ型膠原(Col Ⅱ)酶聯免疫試劑盒(CSB-E08092r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的Col Ⅱ含量。Col Ⅱ即Ⅱ型膠原,是軟骨基質的主要成分。其與關節炎等疾病密切相關,自身抗體識別可觸發免疫反應。研究機制主要圍繞其在軟骨穩態維持、免疫調節中的作用,探索其在疾病診斷、治療靶點方面的價值。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,該產品可廣泛應用于軟骨發育、退行性關節疾病機制研究、藥物或生物材料對軟骨代謝影響的體外評估等科研領域,為探索Col Ⅱ在骨骼系統相關疾病中的作用提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
  • 縮寫:
    Col Ⅱ
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 83-92  
    1:200 Average % 94  
    Range % 90-99  
    1:400 Average % 92  
    Range % 88-96  
    1:800 Average % 102  
    Range % 98-105  
  • 回收率:
    The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 93-96  
    EDTA plasma (n=4) 98 89-104  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.584 2.599 2.592 2.438  
    500 2.217 2.253 2.235 2.081  
    250 1.765 1.792 1.779 1.625  
    125 1.292 1.264 1.278 1.124  
    62.5 0.789 0.773 0.781 0.627  
    31.2 0.396 0.388 0.392 0.238  
    15.6 0.257 0.269 0.263 0.109  
    0 0.153 0.155 0.154    
  • 數據處理:
  • 貨期:
    3-5 working days

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