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  4. Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

  • 中文名稱：
    大鼠心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯免疫試劑盒
  • 貨號：
    CSB-E08594r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯免疫試劑盒（CSB-E08594r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的cTn-I含量。cTn-I（肌鈣蛋白I）是心肌肌肉收縮的調節蛋白，僅在心肌組織中表達，具有高度的心肌特異性和敏感性，是診斷急性心肌梗死（AMI）的首選生化指標。其研究機制主要涉及心肌損傷后cTn-I釋放到血液中，通過檢測血液中cTn-I水平，可以客觀反映心肌損傷情況。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于基礎科研中對心肌損傷機制的研究，支持藥物心臟毒性評估、心肌缺血再灌注損傷研究等實驗場景，滿足實驗室對心血管疾病機制探索、病理模型建立等科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Tnni3 ELISA Kit; Ctni ELISA Kit; Tni ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
  • 縮寫：
    cTn-Ⅰ
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-98  
    1:2 Average % 98  
    Range % 91-104  
    1:4 Average % 99  
    Range % 94-105  
    1:8 Average % 87  
    Range % 82-93  
  • 回收率：
    The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 82-92  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.321 2.220 2.271 2.118  
    1000 1.340 1.441 1.391 1.238  
    500 0.814 0.835 0.825 0.672  
    250 0.529 0.540 0.535 0.382  
    125 0.345 0.334 0.340 0.187  
    62.5 0.261 0.250 0.256 0.103  
    31.25 0.197 0.193 0.195 0.042  
    0 0.153 0.152 0.153 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat cTn-I antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled cTn-I antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展：
    cTn-I（肌鈣蛋白I）是心肌細胞中的一種肌鈣蛋白，在心肌梗死的診斷和治療中具有重要作用。最新研究表明，cTn-I不僅在急性心肌梗死中有顯著升高，也在慢性心力衰竭、冠心病和心肌缺血等其他心血管疾病中存在不同程度的升高。此外，cTn-I還可能是COVID-19患者的疾病評估和治療監測的有用指標。
  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻：
    1. Pim-1 is a novel kinase that phosphorylates cTnI primarily at Ser23/24 and Ser150 in cardiomyocytes, which in turn may modulate myofilament function under a variety of physiological and pathophysiological conditions. PMID: 29544221
    2. Cardiac troponin I mutation P83S present in hypertrophic cardiomyopathy changes the contractile myofibril properties and modulation by PKA-mediated phosphorylation. PMID: 27150586
    3. Troponin I interaction with calcium and Troponin C in cardiac muscle PMID: 28864299
    4. Compared to cTnI(WT), both truncations displayed greater Ca(2+)-sensitivity and faster cross-bridge attachment rates at both SLs. Furthermore, cTnI(1-167) slowed MgADP release rate and enhanced cross-bridge binding. Our findings imply that cTnI-MD truncations affect the blocked-to closed-state transition(s) and destabilize the closed-state position of tropomyosin. PMID: 28958680
    5. PKC phosphorylates cardiac troponin I (cTnI) S23/24, S43/45 and T144 to fine tune myocyte function. PMID: 28587770
    6. These findings demonstrate that S-nitrosylation and S-glutathionylation exert opposing effects on Ca(2+) sensitivity in mammalian FT muscle fibers, mediated by competitive actions on Cys134 of TnIf. PMID: 27974300
    7. the interaction between cTnC and cTnI in skinned papillary muscle strips is dependent on sarcomere length PMID: 26944554
    8. cTnIS43/45N is a functionally conservative substitution, and may be appropriate for use as a phospho-null in rodent models designed for studies on PKC modulation of cardiac performance PMID: 26869200
    9. This study suggests that cTnI point of care tests can accurately determine heat stroke (HS) severity and could serve as simple, portable, cost-effective HS field tests. PMID: 26290107
    10. The present study utilizes viral gene transfer of cTnI with phosphomimetic S43D and/or S45D substitutions to evaluate their individual and combined influences on function in intact adult cardiac myocytes. PMID: 25481661
    11. Results suggest that weakened troponin C interaction with cTnI, via PKA phosphorylation of cTnI, may slow thin filament activation and result in increased myofilament relaxation kinetics PMID: 25185555
    12. The findings elucidate the pathogenesis of MI, and the gradual increase in serum adropin could be a novel diagnostic marker and serve as an alternative to troponin-I measurement for diagnosing MI. PMID: 24932661
    13. molecular determinants of cardiac myocyte performance as conferred by isoform-specific TnI residues PMID: 24853739
    14. Arsenic induced ventricular hypertrophy occurs via MEF2A/CAMKK2/CALM3/TNNI3 signaling. PMID: 25089838
    15. Data indicate that the serum TnI level was significantly greater in the acute coronary syndrome (ACS) group compared to the control group. PMID: 23904327
    16. A cutoff value of 4.8ng/mL for cTnI could be used as early as 8h after MI to accurately identify infarct in this model, whereas echocardiographic images taken 48h after MI predicted the infarcted area 14days after MI. PMID: 23764111
    17. Mutation in the C helix of cTnC can reduce Ca2+ binding affinity and cTnC-cTnI interaction. PMID: 23454346
    18. Hypertrophic cardiomyopathy related mutations R146G/Q and R163W impact interactions between cTnI and cardiac troponin C or actin. PMID: 23246786
    19. Aan increase in calpain activity may enhance cTnI degradation in the myocardium of tail-suspended rats. PMID: 20945043
    20. Normal developing myocardium and skeletal muscle transiently share both sk-fMHC and cTn-I proteins. PMID: 22808244
    21. the functional impact of cardiac troponin I (cTnI) phosphorylation by protein kinase A PMID: 22684024
    22. cTnC, cTnI, cTnT and cTm are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei; significantly, these four proteins appear between days 3 and 5 in both myofilaments and nuclei PMID: 22364878
    23. differential histidine ionization may be necessary for cTnI A164H to act as a molecular sensor capable of modulating sarcomere performance in response to changes in the cytosolic milieu PMID: 22500757
    24. Structural dynamics of C-domain of cardiac troponin I protein in reconstituted thin filament. PMID: 22207765
    25. PKC phosphorylation of cTnI may be maladaptive and potentially associated with cardiac dysfunction PMID: 22052912
    26. Cardiac function and phosphorylation of PLB and cTnI were compared in pacing, isoproterenol treatment, and combined pacing and isoproterenol treatment in isolated working heart. PMID: 21876643
    27. Ulinastatin may protect myocardium from the damage resulted from sepsis in a rat model, probably by lowering expressions of cTnI, TNF-alpha and ET-1. PMID: 20594472
    28. Data show that dual expression of two CM mutants, tropomyosin mutant A63V and cardiac troponin mutant R146G, were shown to additively slow myocyte relaxation beyond either mutant studied in isolation. PMID: 20161772
    29. Time-dependent cTnI breakdown occurs during global ischemia independent of reperfusion. cTnI breakdown during ischemia is further increased in presence of antioxidants. ROS generated during ischemia may play cTnI protective role. PMID: 15142843
    30. cardiac Troponin I Thr144 plays an important role in the acute acceleration of relaxation, whereas Ser23/Ser24 contributes to relaxation during more prolonged activation of protein kinase C by endothelin PMID: 16236710
    31. GATA elements control repression of cTNI promoter activity in skeletal muscle cells. PMID: 17875210
    32. Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. PMID: 18397962
    33. cTnI-R145G expression influences the response to adrenergic stimulation dependent on the receptor subtype PMID: 18548271
    34. Metabolic inhibition of cardiomyocytes induces a parallel release of intact cTnI and its degradation products, starting only after onset of irreversible cardiomyocyte damage. PMID: 18721805
    35. the nNOS-PMCA4b complex regulates contractility via cAMP and phosphorylation of both PLB and cTnI. PMID: 19278978

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  • 蛋白家族：
    Troponin I family
  • 數據庫鏈接：