Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠骨成型蛋白4(BMP-4)酶聯(lián)免疫試劑盒（CSB-E04511r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BMP4含量。BMP4，即骨形態(tài)發(fā)生蛋白4，是TGF-β超家族成員，具有促進成骨、血管生成、胚胎分化和神經干細胞分化等功能。其研究背景涉及心血管疾病、骨再生和腫瘤生長等，機制上通過調節(jié)細胞增殖、分化和遷移等過程發(fā)揮作用。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，本試劑盒適用于科研領域中對BMP - 4功能機制的探索，例如骨骼發(fā)育動態(tài)研究、再生醫(yī)學中組織修復模型的分子機制解析、胚胎發(fā)育過程中形態(tài)發(fā)生調控的定量分析，以及相關疾病模型的分子標志物檢測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
縮寫：

BMP4
Uniprot No.：

Q06826
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-105
1:2	Average %	91
	Range %	85-97
1:4	Average %	86
	Range %	82-90
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

TGF-beta family
數據庫鏈接：

STRING: 10116.ENSRNOP00000012957

UniGene: Rn.10318