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Rat Apoptosis regulator BAX(BAX) ELISA kit

  • 中文名稱:
    大鼠細胞凋亡調節因子BAX(BAX)酶聯免疫試劑盒
  • 貨號:
    CSB-EL002573RA
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠細胞凋亡調節因子BAX(BAX)酶聯免疫試劑盒(CSB-EL002573RA)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BAX含量。BAX是一種重要的促凋亡蛋白。其背景在于它屬于Bcl-2蛋白家族成員。研究機制方面,在細胞受到凋亡刺激時,BAX會從胞質轉位到線粒體膜上,促使線粒體膜通透性改變,釋放細胞色素C等促凋亡物質,進而激活凋亡級聯反應。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,適用于科研場景中細胞凋亡機制研究、藥物誘導的凋亡效應評估或疾病模型(如缺血再灌注損傷、癌癥)中BAX表達水平的動態監測,為探索凋亡相關信號通路提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Bax; Apoptosis regulator BAX
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-92  
    1:2 Average % 96  
    Range % 91-105  
    1:4 Average % 90  
    Range % 87-99  
    1:8 Average % 93  
    Range % 82-98  
  • 回收率:
    The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-97  
    EDTA plasma (n=4) 97 93-105  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.091 1.995 2.043 1.844  
    2000 1.751 1.776 1.764 1.565  
    1000 1.368 1.432 1.400 1.201  
    500 0.985 0.972 0.979 0.780  
    250 0.535 0.544 0.540 0.341  
    125 0.348 0.352 0.350 0.151  
    62.5 0.244 0.237 0.241 0.042  
    0 0.201 0.197 0.199    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
    BAX是一種參與調控細胞凋亡的蛋白質,屬于Bcl-2家族。最近的研究表明,BAX不僅在細胞凋亡中發揮重要作用,而且在多種疾病的發生中也具有重要作用。例如,BAX在癌癥、神經系統疾病、肝臟疾病等的發生中都發揮了重要作用。此外,一些研究還發現BAX在代謝調節、免疫系統調節等方面也具有重要作用。
  • 功能:
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 亞細胞定位:
    [Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.
  • 蛋白家族:
    Bcl-2 family
  • 組織特異性:
    Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
  • 數據庫鏈接:


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