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Rat 4-Hydroxynonenal(HNE)ELISA Kit

  • 中文名稱:
    大鼠4-羥基壬烯酸(HNE)酶聯免疫試劑盒
  • 貨號:
    CSB-EQ027232RA
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠4-羥基壬烯酸(HNE)酶聯免疫試劑盒(CSB-EQ027232RA)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的HNE含量。HNE即人中性粒細胞彈性蛋白酶,在人體免疫防御中起重要作用。但過度激活會引發多種疾病。研究其機制可聚焦在它對底物蛋白的降解、信號通路的調控等方面,以此為靶點有望開發出治療相關病癥如肺部炎癥疾病的藥物。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,可應用于氧化應激相關機制研究、抗氧化藥物干預效果評估,或心血管疾病、代謝綜合征等動物模型中脂質過氧化水平的動態監測,為科研人員提供靈敏、可靠的實驗工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    HNE
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 83-92  
    1:200 Average % 96  
    Range % 89-99  
    1:400 Average % 95  
    Range % 91-100  
    1:800 Average % 93  
    Range % 87-98  
  • 回收率:
    The recovery of rat HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-95  
    EDTA plasma (n=4) 98 94-101  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.118 0.110 0.114    
    500 0.184 0.192 0.188    
    250 0.265 0.273 0.269    
    125 0.474 0.466 0.470    
    62.5 0.625 0.615 0.620    
    31.2 1.129 1.074 1.102    
    15.6 2.182 2.281 2.232    
    0 2.877 2.985 2.931    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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