Rabbit terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit
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中文名稱：兔末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E15838Rb
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：兔末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒（CSB-E15838Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的TCC C5b-9含量。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中補(bǔ)體系統(tǒng)功能評(píng)估、炎癥反應(yīng)機(jī)制探索以及自身免疫性疾病模型的建立，例如在腎臟疾病、動(dòng)脈粥樣硬化或神經(jīng)退行性疾病等領(lǐng)域的實(shí)驗(yàn)設(shè)計(jì)中，可通過(guò)檢測(cè)血清或血漿中的C5b - 9水平，揭示補(bǔ)體終末通路激活程度及其病理學(xué)關(guān)聯(lián)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫：TCC C5b-9
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種屬：Oryctolagus cuniculus (Rabbit)
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樣本類型：serum, plasma
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檢測(cè)范圍：7.8 ng/mL-500 ng/mL
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靈敏度：1.95 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-92 1:2 Average % 96 Range % 91-105 1:4 Average % 97 Range % 92-103 1:8 Average % 91 Range % 86-98 -
回收率：
The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 89-109 EDTA plasma (n=4) 97 92-108 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 500 2.659 2.563 2.611 2.519 250 2.240 2.128 2.184 2.092 125 1.488 1.375 1.432 1.340 62.5 0.845 0.817 0.831 0.739 31.2 0.444 0.434 0.439 0.347 15.6 0.247 0.239 0.243 0.151 7.8 0.156 0.148 0.152 0.060 0 0.093 0.091 0.092 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Differential Interactive Effects of Cartilage Traumatization and Blood Exposure In Vitro and In Vivo Joos H. et al,Am J Sports Med,2015
產(chǎn)品評(píng)價(jià)
微信截圖20230808110042.png)
樣品類型：血清
樣品信息：其他 兔
稀釋比：沒(méi)有稀釋
產(chǎn)品評(píng)價(jià)： 我使用CSB-E15838Rb試劑盒測(cè)定正常兔血清OD值，測(cè)試結(jié)果正常
By 陳老師
樣品類型：血清
樣品信息：其他 兔
稀釋比：沒(méi)有稀釋
產(chǎn)品評(píng)價(jià)： 我用CSB-E15838Rb檢測(cè)正常兔血清中含量，標(biāo)準(zhǔn)曲線的R2可以做到高達(dá)0.9924，標(biāo)準(zhǔn)曲線符合要求，樣品OD值其中之一3.255、3.422，檢測(cè)結(jié)果準(zhǔn)確且完美。
By 王老師
樣品類型：血清
樣品信息：其他 兔
稀釋比：沒(méi)有稀釋
產(chǎn)品評(píng)價(jià)： 我用CSB-E15838Rb檢測(cè)正常兔血清中β-TG含量，標(biāo)準(zhǔn)曲線的R2可以做到高達(dá)0.9978，標(biāo)準(zhǔn)曲線符合要求，樣品OD值其中之一0.250、0.254，可以做到平行，檢測(cè)結(jié)果準(zhǔn)確且完美。
By 張老師
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