Rabbit soluble P-selectin,sP-selectin ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子可溶性P選擇素(sP-selectin)酶聯(lián)免疫試劑盒（CSB-E06861Rb）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液、唾液、組織勻漿樣本中的sP-selectin含量。sP - selectin是一個(gè)受關(guān)注的靶點(diǎn)。它在炎癥、血栓形成等病理生理過程中發(fā)揮重要作用。相關(guān)研究主要聚焦其介導(dǎo)細(xì)胞粘附、調(diào)節(jié)免疫反應(yīng)等機(jī)制，通過抑制或調(diào)控它的活性，有望為治療炎癥性疾病、心血管疾病等開拓新的方向。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，可滿足基礎(chǔ)科研中疾病機(jī)制研究、藥物作用評(píng)估或生物標(biāo)志物探索等需求，尤其適用于炎癥模型、血管功能實(shí)驗(yàn)或代謝相關(guān)研究的體外樣本檢測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

sP-selectin
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, cell culture supernates, urine, saliva, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	95-101
1:40	Average %	96
	Range %	93-99
1:80	Average %	87
	Range %	85-90
1:160	Average %	92
	Range %	87-95

回收率：

The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	101	99-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.