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Rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

  • 中文名稱:
    兔子細胞間粘附分子1(ICAM-1/CD54)酶聯免疫試劑盒
  • 貨號:
    CSB-E06974Rb
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    兔子細胞間粘附分子1(ICAM-1/CD54)酶聯免疫試劑盒(CSB-E06974Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的ICAM-1/CD54含量。ICAM-1/CD54是重要細胞黏附分子。其背景為在多種細胞表面表達,參與免疫應答、炎癥反應等生理病理過程。研究機制上,它能介導細胞間黏附與信號傳導,異常表達與多種疾病如炎癥性疾病、腫瘤轉移等密切相關,是潛在治療靶點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,兔類動物模型中炎癥相關疾病機制研究、免疫調節藥物開發評估、感染病理進程分析、心血管疾病模型、自身免疫性疾病研究、病毒/細菌感染實驗中的生物標志物動態監測本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    ICAM-1/CD54
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:5Average %94
    Range %91-98
    1:10Average %99
    Range %91-106
    1:20Average %87
    Range %82-94
    1:40Average %94
    Range %86-98
  • 回收率:
    The recovery of rabbit ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-96
    EDTA plasma (n=4)10096-106
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.694 2.594 2.644 2.501
    202.144 2.044 2.094 1.951
    101.324 1.284 1.304 1.161
    50.754 0.734 0.744 0.601
    2.50.536 0.546 0.541 0.398
    1.250.351 0.361 0.356 0.213
    0.6250.220 0.230 0.225 0.082
    00.142 0.143 0.143  
  • 數據處理:
  • 貨期:
    3-5 working days

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