Rabbit basic fibroblast growth factor,bFGF ELISA Kit

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說明書下載

中文名稱：

兔子堿性成纖維細胞生長因子(bFGF)酶聯(lián)免疫試劑盒
貨號：

CSB-E06938Rb
規(guī)格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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產(chǎn)品描述：

兔子堿性成纖維細胞生長因子(bFGF)酶聯(lián)免疫試劑盒（CSB-E06938Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF2含量。FGF2即成纖維細胞生長因子2，在細胞的增殖、分化及血管生成等生理過程中發(fā)揮重要作用。其研究機制方面，F(xiàn)GF2通過與細胞膜上的受體結(jié)合，激活下游信號通路，影響細胞行為，在腫瘤、心血管疾病等研究領(lǐng)域受關(guān)注。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于體外培養(yǎng)細胞模型、動物組織樣本或體液樣本的bFGF含量檢測，為研究組織再生機制、腫瘤微環(huán)境調(diào)控或炎癥反應(yīng)等科研領(lǐng)域提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FGF2 ELISA Kit; Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Fragment ELISA Kit
縮寫：

FGF2
Uniprot No.：

P48799
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	104
	Range %	100-108
1:4	Average %	85
	Range %	81-89
1:8	Average %	99
	Range %	95-102

回收率：

The recovery of rabbit bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	97	94-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	1.863	1.812	1.838	1.679
250	1.699	1.598	1.649	1.490
125	1.428	1.436	1.432	1.273
62.5	1.115	1.168	1.142	0.983
31.2	0.804	0.831	0.818	0.659
15.6	0.563	0.574	0.569	0.410
7.8	0.362	0.372	0.367	0.208
0	0.159	0.158	0.159

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
亞細胞定位：

Secreted. Nucleus.
蛋白家族：

Heparin-binding growth factors family
數(shù)據(jù)庫鏈接：

STRING: 9986.ENSOCUP00000000416

UniGene: Ocu.1874

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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