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Rabbit Epidermal growth factor,EGF ELISA Kit

  • 中文名稱:
    兔子表皮生長因子(EGF)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E06909Rb
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔子表皮生長因子(EGF)酶聯(lián)免疫試劑盒(CSB-E06909Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的EGF含量。表皮生長因子(EGF)是一種重要的生長因子,屬于表皮生長因子家族,通過與其受體EGFR結(jié)合,激活細(xì)胞內(nèi)的信號傳導(dǎo)途徑,促進細(xì)胞增殖、分化、遷移和存活。EGF在胚胎發(fā)育、組織再生和傷口愈合中發(fā)揮關(guān)鍵作用,同時其異常表達與多種疾病,尤其是癌癥的發(fā)生發(fā)展密切相關(guān)。試劑盒檢測范圍為0.5 ng/mL-8 ng/mL,特別適用于探究EGF在皮膚屏障功能、消化道黏膜修復(fù)、創(chuàng)傷愈合等研究領(lǐng)域的調(diào)控機制。科研人員可利用分析不同生理/病理條件下體液和組織中的EGF動態(tài)變化,為研究生長因子在再生醫(yī)學(xué)、腫瘤微環(huán)境中的作用提供量化依據(jù),其寬檢測范圍設(shè)計可有效應(yīng)對復(fù)雜生物樣本中目標(biāo)蛋白濃度的顯著波動。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.5 ng/mL-8 ng/mL
  • 靈敏度:
    0.25 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %111
    Range %100-115
    1:2Average %92
    Range %84-97
    1:4Average %100
    Range %90-104
    1:8Average %100
    Range %93-104
  • 回收率:
    The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9287-97
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    80.147 0.141 0.144
    40.311 0.322 0.317
    20.501 0.534 0.518
    10.926 0.902 0.914
    0.51.932 1.966 1.949
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

相關(guān)問答

 常見問題解答
Q:

Can I check the ingredients of Stop solution for CSB-E06909Rb.
Please also advise if the standard is derived from CHO or E.coli.

A:
Thanks for your inquiry. Standard: CHO-derived recombinant protein
Stop solution: 4N sulfuric acid
Q:

Can you please also tell me if the standard is bacurovirus derived? We don't need the sequence, just the source.

A:
Thanks for your inquiry.The standard is not bacurovirus derived. It is CHO-derived recombinant protein.
Q:

I'd like to know the necessary sample volume for this ELISA kit.

A:
Thanks for your inquiry.The sample loading volume is 50ul per well.


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