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  4. Pig hepatocyte growth factor,HGF ELISA Kit

Pig hepatocyte growth factor,HGF ELISA Kit

  • 中文名稱：
    豬肝細胞生長因子(HGF)酶聯免疫試劑盒
  • 貨號：
    CSB-E06795p
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    豬肝細胞生長因子(HGF)酶聯免疫試劑盒（CSB-E06795p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HGF含量。HGF（肝細胞生長因子）是一種多功能細胞因子，在胚胎發育、組織再生與修復等生理過程中發揮關鍵作用。其異常表達與腫瘤生長、侵襲和轉移密切相關。研究機制主要是通過與受體c-Met結合，激活下游信號通路，影響細胞的增殖、存活等。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，可廣泛應用于肝臟再生機制研究、疾病模型（如肝纖維化或損傷修復）中HGF動態變化的檢測，以及藥物或生物制劑干預后HGF調控效應的評估，為相關基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %84-92
    1:2Average %104
    Range %94-110
    1:4Average %95
    Range %89-99
    1:8Average %94
    Range %86-97
  • 回收率：
    The recovery of pig HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9290-95
    EDTA plasma (n=4)9792-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.894 2.794 2.844 2.683
    20002.344 2.244 2.294 2.133
    10001.524 1.474 1.499 1.338
    5000.954 0.924 0.939 0.778
    2500.556 0.546 0.551 0.390
    1250.371 0.361 0.366 0.205
    62.50.240 0.230 0.235 0.074
    00.162 0.160 0.161 　
  • 數據處理：
  • 貨期：
    3-5 working days

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