Pig growth hormone,GH ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬生長激素(GH)酶聯(lián)免疫試劑盒（CSB-E06813p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 基因編碼生長激素，生長激素在人體生長發(fā)育、代謝調(diào)節(jié)等方面至關(guān)重要。其相關(guān)研究機制圍繞生長激素的分泌調(diào)節(jié)、信號通路、功能作用等展開。如對其分泌調(diào)控機制的研究，有助于了解生長發(fā)育異常疾病，為臨床治療提供思路。試劑盒檢測范圍為2.5 ng/mL-100 ng/mL，適用于動物生理學(xué)、營養(yǎng)代謝研究及生長調(diào)控機制探索等科研領(lǐng)域。可為畜牧養(yǎng)殖優(yōu)化、飼料效能評估等研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
縮寫：

GH1
Uniprot No.：

P01248
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.5 ng/mL-100 ng/mL
靈敏度：

2.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	95
	Range %	89-100
1:4	Average %	94
	Range %	88-99
1:8	Average %	95
	Range %	90-103

回收率：

The recovery of pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	98	92-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數(shù)據(jù)庫鏈接：

STRING: 9823.ENSSSCP00000018314

UniGene: Ssc.10492