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  4. Pig coagulation factor VIII(FVIII) ELISA Kit

Pig coagulation factor VIII(FVIII) ELISA Kit

  • 中文名稱：
    豬凝血因子VIII(FVIII)酶聯免疫試劑盒
  • 貨號：
    CSB-E15000p
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    豬凝血因子VIII(FVIII)酶聯免疫試劑盒（CSB-E15000p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F8含量。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于出血性疾病機制研究、凝血調節藥物開發、動物模型凝血功能評估等科研領域，特別適用于畜牧科學、獸醫學研究及生物制藥領域的凝血相關實驗分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    F8 ELISA kit; CF8Coagulation factor VIII ELISA kit; Procoagulant component ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 ng/mL-4000 ng/mL
  • 靈敏度：
    15.62 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig FVIII in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    89

    Range %

    84-95

    1:200

    Average %

    94

    Range %

    89-99

    1:400

    Average %

    93

    Range %

    86-100

    1:800

    Average %

    98

    Range %

    91-103

  • 回收率：

    The recovery of pig FVIII spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    90-101

    EDTA plasma (n=4)

    89

    84-94

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    4000

    0.134

    0.128

    0.131

    2000

    0.221

    0.237

    0.229

    1000

    0.384

    0.398

    0.391

    500

    0.615

    0.629

    0.622

    250

    0.868

    0.881

    0.875

    125

    1.214

    1.253

    1.234

    62.5

    1.649

    1.684

    1.667

    0

    2.236

    2.125

    2.181

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • 基因功能參考文獻：
    1. It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID: 28237345
    2. Report Factor VIII organisation on nanodiscs with different lipid composition. PMID: 25589466
    3. thrombin stimulates transglutaminase activity in articular cartilage by directly cleaving factor XIII and by receptor-mediated up-regulation of factor XIII synthesis PMID: 14983233
    4. cupredoxin-like A1 subdomains in fVIII contain inter-species differences that are a result of selective pressure on the dissociation rate constant PMID: 16513639
  • 亞細胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Multicopper oxidase family
  • 數據庫鏈接：


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