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Pig apolipoprotein A1 (Apo-A1) ELISA Kit

  • 中文名稱:
    豬載脂蛋白A1(Apo-A1)酶聯免疫試劑盒
  • 貨號:
    CSB-E06834p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    豬載脂蛋白A1(Apo-A1)酶聯免疫試劑盒(CSB-E06834p)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是一種位于細胞膜外的分泌蛋白,是高密度脂蛋白(HDL)的主要結構載脂蛋白,在逆向膽固醇轉運途徑中起關鍵作用。其與細胞表面受體SR-BI相互作用,促進膽固醇從組織到肝臟的逆向運輸,具有抗炎、抗動脈粥樣硬化等作用。研究顯示,APOA1與病毒感染、動脈粥樣硬化、糖尿病、神經系統疾病、腫瘤等疾病有關。試劑盒檢測范圍為80 μg/mL-2000 μg/mL,適用于豬脂代謝機制研究、心血管疾病動物模型評估、營養干預對脂蛋白代謝的影響分析等科研領域,為豬相關代謝性疾病的基礎研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    80 μg/mL-2000 μg/mL
  • 靈敏度:
    50 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %89-103
    1:2Average %90
    Range %85-103
    1:4Average %93
    Range %88-100
    1:8Average %89
    Range %82-97
  • 回收率:
    The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9996-104
    EDTA plasma (n=4)9086-95
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    20000.342 0.348 0.345
    8000.533 0.483 0.508
    4800.682 0.657 0.670
    2001.021 0.987 1.004
    801.267 1.325 1.296
    01.903 1.954 1.929
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能參考文獻:
    1. Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID: 25377528
    2. blood level decreases during experimentally induced acute-phase processes in pigs PMID: 15845530
    3. down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID: 15878672
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Apolipoprotein A1/A4/E family
  • 組織特異性:
    Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.
  • 數據庫鏈接:

    KEGG: ssc:397691

    STRING: 9823.ENSSSCP00000015994

    UniGene: Ssc.807



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