Pig Myeloperoxidase (MPO) ELISA kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬髓過(guò)氧化物酶(MPO)酶聯(lián)免疫試劑盒（CSB-E09397p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MPO含量。MPO即髓過(guò)氧化物酶，是一種存在于中性粒細(xì)胞和單核細(xì)胞中的血紅素蛋白。它在炎癥反應(yīng)和免疫防御中起重要作用，可參與殺滅病原體。其研究機(jī)制聚焦于其在氧化應(yīng)激、炎癥損傷等病理過(guò)程中的作用，有望為相關(guān)疾病治療提供新方向。試劑盒檢測(cè)范圍為15.6 ng/mL-1000 ng/mL，適用于科研領(lǐng)域中對(duì)感染性疾病模型、免疫調(diào)控機(jī)制、心血管或神經(jīng)退行性疾病的病理研究，也可用于評(píng)估抗炎藥物或抗氧化劑對(duì)MPO活性的干預(yù)效果。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

MPO
Uniprot No.：

K7GRV6
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	87-99
1:10	Average %	97
	Range %	93-105
1:20	Average %	85
	Range %	82-89
1:40	Average %	90
	Range %	85-98

回收率：

The recovery of pig MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	95	89-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.