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Mouse platelet-derived growth factor-BB,PDGF-BB ELISA Kit

  • 中文名稱:
    小鼠血小板衍生生長(zhǎng)因子BB(PDGF-BB)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08925m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠血小板衍生生長(zhǎng)因子BB(PDGF-BB)酶聯(lián)免疫試劑盒(CSB-E08925m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PDGF-BB含量。PDGF-BB 是血小板衍生生長(zhǎng)因子異構(gòu)體。其背景在于它在細(xì)胞生長(zhǎng)、增殖等生理過(guò)程有關(guān)鍵作用。研究機(jī)制主要圍繞其與受體結(jié)合,激活下游信號(hào)通路,在腫瘤血管生成、纖維化疾病發(fā)展等病理過(guò)程有重要影響,也是藥物研發(fā)熱門靶點(diǎn)。試劑盒檢測(cè)范圍為0.18 ng/mL-12 ng/mL,科研人員可將其應(yīng)用于小鼠模型中的炎癥反應(yīng)研究、血管生成機(jī)制探索或組織再生相關(guān)實(shí)驗(yàn),也可用于評(píng)估藥物干預(yù)或基因修飾對(duì) PDGF-BB 表達(dá)的影響。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫:
    PDGF-BB
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.18 ng/mL-12 ng/mL
  • 靈敏度:
    0.047 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %82-88
    1:2Average %94
    Range %89-98
    1:4Average %104
    Range %97-109
    1:8Average %99
    Range %93-105
  • 回收率:
    The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-101
    EDTA plasma (n=4)8982-96
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    122.389 2.501 2.445 2.281
    61.445 1.529 1.487 1.323
    30.905 0.984 0.945 0.781
    1.50.656 0.633 0.645 0.481
    0.750.345 0.349 0.347 0.183
    0.370.287 0.265 0.276 0.112
    0.180.223 0.218 0.221 0.057
    00.159 0.169 0.164
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)



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