Mouse mitochondrial respiratory chain complex I ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠線粒體呼吸鏈復(fù)合物I酶聯(lián)免疫試劑盒（CSB-EQ027280MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的MRCC-I含量。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，本試劑盒采用高特異性配對(duì)抗體，通過(guò)標(biāo)準(zhǔn)化的預(yù)包被板與顯色系統(tǒng)實(shí)現(xiàn)目標(biāo)蛋白的定量分析，適用于基礎(chǔ)研究中線粒體功能評(píng)估、疾病模型構(gòu)建（如心肌缺血、阿爾茨海默癥等）或藥物干預(yù)后能量代謝變化的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

MRCC-I
種屬：

Mus musculus (Mouse)
樣本類(lèi)型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRCC-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-100
1:2	Average %	100
	Range %	94-103
1:4	Average %	94
	Range %	84-98
1:8	Average %	100
	Range %	93-103

回收率：

The recovery of mouse MRCC-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-97
EDTA plasma (n=4)	99	91-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.