国产午夜福利片1000无码丨人妻国产成人久久av免费高清丨午夜做受视频试看6次丨日韩av不卡一区在线免费观看丨在线观看精品三级欧美

Your Good Partner in Biology Research

Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

  • 中文名稱:
    小鼠白介素18結合蛋白(IL-18BP)酶聯免疫試劑盒
  • 貨號:
    CSB-E17797m
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠白介素18結合蛋白(IL-18BP)酶聯免疫試劑盒(CSB-E17797m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液樣本中的IL-18BP含量。IL-18BP(白介素-18結合蛋白)是一種免疫檢查點蛋白,可抑制IL-18與其受體結合,調節Th1細胞因子反應。其在多種腫瘤和自身免疫性疾病中高表達,研究機制涉及IL-18與IL-18BP的相互作用及其對免疫反應的影響。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,科研人員可將其應用于炎癥性疾病動物模型的機制研究、藥物干預效果評估或體外細胞實驗中IL-18/IL-18BP信號通路的動態監測,特別適用于探索類風濕性關節炎、炎癥性腸病等病理過程中IL-18通路的調控機制本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit
  • 縮寫:
    IL-18BP
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, cell culture supernates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %93-100
    1:2Average %84
    Range %80-88
    1:4Average %104
    Range %100-107
    1:8Average %91
    Range %87-95
  • 回收率:
    The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10097-103
    EDTA plasma (n=4)8984-95
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.729 2.799 2.764 2.592
    52.302 2.342 2.322 2.150
    2.51.513 1.587 1.550 1.378
    1.250.928 0.965 0.947 0.775
    0.6250.548 0.539 0.544 0.372
    0.3120.381 0.375 0.378 0.206
    0.1560.278 0.274 0.276 0.104
    00.171 0.173 0.172
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 3 個評價

樣品類型:組織勻漿液 小鼠結腸

樣品信息:小鼠

稀釋比:其他 稀釋2倍

產品評價: 我用了貨號為CSB-E17797m的Mouse Interleukin-18 Binding Prorein (IL-18BP)ELISA Kit檢測了DSS誘導結腸炎之后小鼠結腸組織勻漿中的IL-18BP蛋白,OD值為2左右,該試劑盒效果不錯,反應靈敏,達到了預期實驗的目的

By 胡老師

樣品類型:血清

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我用CSB-E17797m檢測正常小鼠和造模狼瘡小鼠的血清,未稀釋樣品進行檢測。標準品OD450值最高為1.99,最低為0.02,標準曲線R平方大于0.99。小鼠血清OD450值范圍約為0.3-1.3左右, 對應濃度為1.5-7ng/mL, 符合我的預期濃度范圍。

By 唐老師

樣品類型:血漿(抗凝劑) EDTA

樣品信息:小鼠

稀釋比:其他 1:5

產品評價: 我用E17797m的IL18BP ELISA試劑盒檢測小鼠血漿IL18BP濃度,1:5倍稀釋,OD值為0.313

By 許老師

靶點詳情

  • 功能:
    Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
  • 基因功能參考文獻:
    1. IL-18BP ameliorates cardiac ischemia/reperfusion injury in part through suppression of Th17 differentiation. PMID: 26340315
    2. Data show that IL-18bp acts as a sensor for IFN-gamma and can regulate both Th1 and Th17 responses in the CNS. PMID: 20644165
    3. Administratioan of murine IL-18BP at the time of the booster injection of bovine CII reduces the progressive severity of collagen-induced arthritis; both clinical disease activity and histological scores are decreased by 50%. PMID: 12574381
    4. functional polarization of Ag-specific CD4(+) Th2 cells entering the CNS during the accelerating phase of experimental autoimmune encephalomyelitis is flexible and dependent on IL-18, which is overturned by IL-18 binding protein PMID: 15778395
    5. IL-18 and IL-18BP may have roles in testicular development, and also in the regulation of testicular functions under physiological conditions PMID: 18299271
    6. A single intravenous dose of recombinant mouse IL18BP decreased neointimal hyperplasia and improved arterial L/A ratios in an atherosclerotic balloon-injury animal model. PMID: 18455646

    顯示更多

    收起更多

  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:


主站蜘蛛池模板: 六盘水市| 宜昌市| 工布江达县| 年辖:市辖区| 岗巴县| 尉犁县| 黎城县| 华蓥市| 改则县| 台东市| 南雄市| 寿光市| 万载县| 盘山县| 丘北县| 延津县| 贵德县| 宣武区| 南华县| 基隆市| 五台县| 贺兰县| 九龙坡区| 达拉特旗| 龙里县| 台前县| 顺平县| 新平| 思南县| 兖州市| 乌拉特中旗| 成安县| 府谷县| 大庆市| 彩票| 盐城市| 古田县| 唐山市| 扎赉特旗| 饶河县| 云龙县|