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  4. Mouse eosinophil cationic protein,ECP ELISA Kit

Mouse eosinophil cationic protein,ECP ELISA Kit

  • 中文名稱：
    小鼠嗜酸性粒細胞陽離子蛋白(ECP)酶聯免疫試劑盒
  • 貨號：
    CSB-E11799m
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠嗜酸性粒細胞陽離子蛋白(ECP)酶聯免疫試劑盒（CSB-E11799m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的ECP含量。ECP即嗜酸性粒細胞陽離子蛋白，在過敏等多種免疫相關疾病背景下發揮作用。研究機制方面，它具有細胞毒性，可破壞寄生蟲、腫瘤細胞等，還能趨化炎癥細胞，參與炎癥反應的調控，在疾病發生發展中扮演重要角色，是潛在治療靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研場景中評估疾病模型（如哮喘、過敏或寄生蟲感染）的嗜酸性粒細胞激活程度，支持多種樣本類型的平行檢測，為研究 Th2 型免疫反應、組織炎癥機制及藥物干預效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    ECP
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-98
    1:2Average %97
    Range %92-106
    1:4Average %93
    Range %88-99
    1:8Average %89
    Range %83-95
  • 回收率：
    The recovery of mouse ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9589-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.546 2.652 2.599 2.435
    52.074 2.076 2.075 1.911
    2.51.413 1.504 1.459 1.295
    1.250.676 0.735 0.706 0.542
    0.6250.486 0.524 0.505 0.341
    0.3120.384 0.359 0.372 0.208
    0.1560.251 0.259 0.255 0.091
    00.163 0.165 0.164 　
  • 數據處理：
  • 貨期：
    3-5 working days

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