Mouse catestatin ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠兒茶酚抑素酶聯(lián)免疫試劑盒（CSB-E17357m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的catestatin含量。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于體外培養(yǎng)細(xì)胞模型中CST分泌水平的動(dòng)態(tài)監(jiān)測(cè)、疾病動(dòng)物模型（如高血壓或心肌缺血模型）組織內(nèi)源性CST表達(dá)分析，以及探索CST在代謝綜合征相關(guān)機(jī)制中的作用，為神經(jīng)內(nèi)分泌調(diào)控、心血管病理機(jī)制等基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

catestatin
種屬：

Mus musculus (Mouse)
樣本類(lèi)型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Biochemicals
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
Range %	87-96
1:2	Average %	99
Range %	95-103
1:4	Average %	101
Range %	94-106
1:8	Average %	95
Range %	89-98

回收率：

The recovery of mouse catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-98
EDTA plasma (n=4)	99	92-106

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.