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Mouse anti-thyroid-globulin antibody,TGAB ELISA Kit

  • 中文名稱:
    小鼠抗甲狀腺球蛋白抗體(ATGA/TGAB)酶聯免疫試劑盒
  • 貨號:
    CSB-E09543m
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠抗甲狀腺球蛋白抗體(ATGA/TGAB)酶聯免疫試劑盒(CSB-E09543m)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的TG Ab含量。TG Ab即甲狀腺球蛋白抗體,是自身免疫性甲狀腺疾病患者血清中常見的自身抗體。其背景與自身免疫紊亂相關。研究機制上,它可能通過破壞甲狀腺濾泡細胞、影響甲狀腺球蛋白代謝等,參與自身免疫性甲狀腺炎的發病過程,影響甲狀腺功能。試劑盒檢測范圍為1.25 U/mL-20 U/mL,適用于血清、血漿樣本的科研分析;在動物模型中常被用于研究甲狀腺自身免疫反應的病理機制及藥物干預效果評估;適用于實驗室環境下的小鼠模型研究,包括甲狀腺自身免疫疾病發病機理探索、免疫調節藥物作用效果驗證以及相關生物標志物動態監測等科研場景本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    TG Ab
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    1.25 U/mL-20 U/mL
  • 靈敏度:
    1.25 U/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 85-101    
    1:2 Average % 89    
    Range % 85-98    
    1:4 Average % 92    
    Range % 84-100    
    1:8 Average % 95    
    Range % 90-100    
  • 回收率:
    The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-103    
    EDTA plasma (n=4) 95 90-105    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average      
    20 0.146 0.151 0.149      
    10 0.278 0.281 0.280      
    5 0.457 0.463 0.460      
    2.5 0.854 0.870 0.862      
    1.25 1.865 1.828 1.847      
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 2 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我用CSB-E09543m檢測正常小鼠血清,其OD為0.5003,模型組OD為0.1912

By 王老師

樣品類型:血清

樣品信息:大鼠

稀釋比:其他 1:10

產品評價: 我用CSB-E09543m檢測自身免疫性甲狀腺炎大鼠,稀釋比例為1:10,用小鼠盒子檢測大鼠,實驗結果也較為準確,符合趨勢。期望能出大鼠TGAB的盒子,我們實驗室需求量很大。

By 丁老師